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One-Plasmid Systems for Blue-light-regulated Gene Expression in Prokaryotes


18.08.2011
| Ref.Nr. 11043
Life Science › Pharma&Biotech

Background
Protein expression systems are very widely used in the life sciences, biotechnology and medicine. Traditional strategies for recombinant protein expression involve the transformation of a gene expression vector into prokaryotic expression hosts and culturing the cells so that they express the desired protein. Using an inducible expression cassette the onset of the protein expression can be controlled by several inducer (f.e. chemical, temperature, cold).

Technology
We offer two compact plasmids for facile light regulated expression of target proteins in prokaryotic hosts. Dependent from the application, one plasmid can be used for light activated and one for light inactivated gene expression. Both plasmids are working without exogenous addition of chromophores or expensive chemical inducers and allow a specific, spatially- and temporally precise control of protein expression. A competitive advantage is the use of light as inducer especially for the production of recombinant proteins. Another advantage is the very precise control of protein expression which is very relevant for several research applications. By using standard chemical inducers a spatially and temporally precise control of protein expression is not possible.

IP Rights

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Origin
Humboldt University, Berlin
Application Area:
Production of proteins in prokaryotes Analysis of protein functions in prokaryotes Pulsed induction of protein expression for several research applications
Development Stage: Product
Weitere Kategorien: Life Science, Pharma & Biotech
Bild des Benutzers Dr. Bettina Büttner
Licensing Manager: Dr. Bettina Büttner
T +49 30 2125 4835
F +49 30 2125 4822
Suitable Industry: Biotechnology and pharmaceutical industry, Research & Development
Benefits:
  • One plasmid is designed for light inactivated and one for light activated gene expression
  • Both plasmids are compact and ready-to-use
  • No exogenous addition of chromophores or chemical inducers is required
  • System facilitates pulsed and/or automated induction of protein expression
  • Allows specific, spatially- and temporally-precise control of protein expression
  • Reduced production costs, because expensive chemical inducers are not required